久久婷婷五月综合色欧美-92久久精品一区二区-亚洲一区二区观看播放-国产区精品系列在线观看-av老司机亚洲精品天堂

【文獻標題】Hepatic Stellate Cells Secreted Hepatocyte Growth Factor Contributes to the Chemoresistance of Hepatocellular Carcinoma

【作者】Guofeng Yu，Yingying Jing，Xingrui Kou，et.al

【作者單位】第二軍醫大學（The Second Military Medical University）

【文獻中引用產品】

人肝細胞生長因子（HGF）ELISA試劑盒

人轉化生長因子β（TGF- β）ELISA試劑盒

【DOI】doi:10.1371/journal.pone.0073312

【影響因子(IF)】3.02

【出版期刊】《PLOS ONE》

【產品原文引用】

Conditioned medium and ELISA

Cultured media (CM) were collected from LX-2 cells cultured in 2D culture dishes and centrifuged at 1000g for 5 minutes to obtain the supernatant. LX-2 CM was incubated with HGF antibody (GTX10678, 0.5 µg/ml) for 24h at 4oC to obtain HGFdepleted LX-2 CM. The level of HGF and TGF-β in CM waere determined by using human HGF ELISA kit(HY10158E,Shanghai HengYuan Biological Technology Co., Ltd) and TGF-β ELISA kit (HY10108E, Shanghai HengYuan Biological Technology Co., Ltd) according to manufacturer’s instructions.

完整版PDF文獻請咨詢在線客服或者聯系我司業務員！

更多公司福利請關注“恒遠生物”VX公眾號！