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【文獻標題】Improved utilization of soybean meal through fermentation with commensal Shewanella sp. MR-7 in turbot (Scophthalmus maximus L.)

【作者】Chaoqun Li，Beili Zhang，Xin Wang，et.al

【作者單位】中國海洋大學（Ocean University of China）

【文獻中引用產品】

魚蛋白酶（Protease） ELISA試劑盒

【關鍵詞】Turbot, Shewanella sp. MR-7, Soybean meal, Fermentation, Intestinal health, Intestinal microbiota

【DOI】doi.org/10.1186/s12934-019-1265-z

【影響因子(IF)】7.40

【出版期刊】《Microbial Cell Factories》

【產品原文引用】

Parameter optimization of Shewanella sp. MR?7

Te isolated strain of Shewanella sp. MR-7 was cultured in 2216E medium (Qingdao Haibo) and shaken at 220 rpm at 25, 30, 35, 37 and 40 °C to determine the efects of temperature on the growth of Shewanella sp. MR-7. Te Shewanella sp. MR-7 was also cultured in 2216E medium at pH 5.0, 6.0, 7.0, 8.0, 9.0 to examine the efect of pH on its growth. Te OD600 of the culture was taken periodically. Te infuences of temperature and pH on the production of protease by Shewanella sp. MR-7 were determined by enzyme-linked immunosorbent assay (ELISA) using protease ELISA kits (HengYuan Biological Technology Co., Ltd, Shanghai, China).

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